Characteristics of glutathione biosynthesis by freshly isolated rat kidney cells.

Characteristics of glutathione biosynthesis by freshly isolated rat kidney cells.

Suspensions of freshly isolated rat kidney cells were used to study glutathione biosynthesis and utilization for amino acid translocation. Incubation of cells isolated from diethylmaleate-perfused kidneys in the presence of glutamate, glycine, and cystine led to replenishment of cellular glutathione and to accumulation of glutathione disulfides in the medium. Cystine could be replaced by cystei...

Binding and uptake of surfactant protein D by freshly isolated rat alveolar type II cells.

Alveolar type II cells secrete, internalize, and recycle pulmonary surfactant, a lipid and protein complex that increases alveolar compliance and participates in pulmonary host defense. Surfactant protein (SP) D, a collagenous C-type lectin, has recently been described as a modulator of surfactant homeostasis. Mice lacking SP-D accumulate surfactant in their alveoli and type II cell lamellar bo...

Fas-mediated cytotoxicity by freshly isolated natural killer cells

The expression of Fas ligand on natural killer (NK) cells and Fas-mediated cytotoxicity by NK cells was investigated. Fas ligand mRNA was expressed in freshly isolated NK cells but not in T cells. Furthermore, the Fas ligand was detected on the cell surface of NK cells by staining with soluble Fas molecule. We analyzed the cytolytic activity of NK cells against thymocyte targets from normal and...

Direct Action of Helicobacter pylori on the Freshly Isolated Rat Gastric Mucosa Cells (GMCs)

Biosynthesis of cytochrome c oxidase by isolated rat liver mitochondria.

When isolated mitochondria which have been labeled with [3H]leucine are solubilized and treated with anti-serum specific for cytochrome c oxidase, labeled polypeptides which correspond to the three largest polypeptides of this enzyme are immunoprecipitated. This indicates that the three largest polypeptides of cytochrome c oxidase which have Mr of 66,000, 39,000, and 23,000 are synthesized by i...